Elucidating the role of muscarinic receptors in psychosis
The intensity readings from each image were then converted to fmol/mg estimated tissue equivalents by comparing the reading from each region to a standard curve of standardized radioactivity on calibrated microscales.
Specific binding was then calculated by subtracting the average non-specific binding value from total binding.
Of the 38 people with Sz, 18 people were diagnosed as having Sz but were part of the subgroup that had levels of cortical CHRM1 equivalent to that observed in control subjects (CHRM Sz) whilst 20 people had CHRM-Sz.
Finally, for each case, PMI was calculated as the time between witnessed death and autopsy or the midpoint between the subject being found and being last seen alive and autopsy.
While we have previously published data on the selectivity of [H]pirenzepine binding under the assay conditions we developed (Scarr & Dean, 2008), we wanted to continue to develop a better understanding of the selectivity of different radioligands for CHRMs.
H]pirenzepine binding in Brodmann's area (BA) 9 from a subset of people with schizophrenia was predictive of decreased muscarinic receptors in other central nervous system (CNS) regions.
Our data show that, under the conditions used, [H]pirenzepine binding in BA 9 from a subset of people with schizophrenia is predictive of decreases in muscarinic receptors in other CNS regions.